Composite

Part:BBa_K1767010

Designed by: naseeb saida   Group: iGEM15_Technion_HS_Israel   (2015-09-16)
Revision as of 16:52, 16 September 2015 by Naseeb s (Talk | contribs)

P(Lac)IQ RBS LuxR ter ter luxpR RBS eyfp ter ter


We designed and built a kill switch mechanism that based on the activation of a lethal gene downstream of an inducible promoter that can be activated over time.

The here presented BioBrick similar to BBa_k1767009 but without Aiia which consist of a constitutive promoter(I14032) driving expression of LuxR, followed by Lux promoter(R0062) . This promoter is activated by LuxR complex driving expression of YFP.

This BioBrick built for testing because we want to see how the first part of our system works without Aiia .

The scheme below explains how this BioBrick works :

Technion HS Israel K1767010.jpg

We performed an experiment to test this part, and here is the result:


Technion HS Israel K1767010 2.png

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 987


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