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Part:BBa_K1709003:Design

Designed by: Astrid Deryckere   Group: iGEM15_KU_Leuven   (2015-09-16)
Revision as of 17:26, 18 September 2015 by AstridD (Talk | contribs) (→‎Design Notes)

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CheZ-GFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1307


Design Notes

1 bp in BBa_K629003 was adapted to create an original restriciton site in the plasmid. CheZ was fused to GFP (BBa_K082003) and its LVA tag was codon-optimized to avoid introducing artificial DNA repetitions. gBlocks were used to partly design the plasmid and the ribosome binding site sequence was based purely on the Anderson library and thus, there is a mistake in the composite part sequence since the scar is only partly present.

Source

DNA sequences originate from the E. Coli genome and previous biobrick sequences. The ribosome binding site was chosen from the Anderson RBS family.

References