Regulatory

Part:BBa_K1679004:Design

Designed by: Qikai Qin   Group: iGEM15_OUC-China   (2015-09-16)
Revision as of 13:51, 16 September 2015 by QinQikai (Talk | contribs) (→‎References)


J23101 and RiboJ


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We want to make a promoter part containing RiboJ to buffer synthetic circuits from genetic context. We synthesized RiboJ with EXSP site and use 3A Assembly to construct this part.


Source

BBa_J23101 We synthesized RiboJ. Our reference is Lou C, Stanton B, Chen Y J, et al. Ribozyme-based insulator parts buffer synthetic circuits from genetic context[J]. Nature biotechnology, 2012, 30(11): 1137-1142.

References

Lou C, Stanton B, Chen Y J, et al. Ribozyme-based insulator parts buffer synthetic circuits from genetic context[J]. Nature biotechnology, 2012, 30(11): 1137-1142.