Part:BBa_K1859001
The 3´side of the intron [BBa_K1332005] +GGSGGS linker
BBa_K1859001 is the 3'side of intron [BBa_K1332005] conjuncted linker to keep folding of proteins. Circular Parts, the 3'side of intron [BBa_K1332005] and the 5'side of intron [BBa_K1332003] , is what use sequence subcloned ribozyme involved self‐splicing in T4 Phage. By associating Circular Parts on both ends of optional sequence, you can circularize mRNA making its DNA.
As for details, refer to iGEM Gifu 2014
In the study of iGEM Gifu 2014, we made long-chain protein from cyclic mRNA, but it lost functionality. It is thought that folding of the protein failed for a cause． Therefore we designed some linkers to fix the folding of the protein. BBa_K1859001 includes sequence of the 3'side of intron [BBa_K1332005] and linker． The linker comes to be in the Circular mRNA when mRNA is circularized. To make this device, we coupled the sequence of the linker, GGSGGS , to the down stream of 3'side of the intron [BBa_K1332005] .
The linker designed elsewhere is here .
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