Part:BBa_K1688015

Nah7 - Upper naphthalene breakdown pathway (inc RBS)

Upper naphthalene breakdown pathway from the Nah7 plasmid of Pseudomonas putida + RBS B0034. Operon consists of ten genes (NahAa, NahAb, NahAc, NahAd, NahB, NahC, NahD, NahE, NahF, NahQ) which in turn codes for seven enzymes that catalyse the breakdown of naphthalene into salicylate.

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal PstI site found at 1884
  Illegal PstI site found at 5879
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 4139
  Illegal PstI site found at 1884
  Illegal PstI site found at 5879
  Illegal NotI site found at 8182
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 5232
  Illegal BamHI site found at 3217
  Illegal BamHI site found at 5919
  Illegal XhoI site found at 1328
  Illegal XhoI site found at 3716
  Illegal XhoI site found at 3868
  Illegal XhoI site found at 9370
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal PstI site found at 1884
  Illegal PstI site found at 5879
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal PstI site found at 1884
  Illegal PstI site found at 5879
  Illegal NgoMIV site found at 1821
  Illegal NgoMIV site found at 3596
  Illegal NgoMIV site found at 5470
  Illegal NgoMIV site found at 6082
  Illegal NgoMIV site found at 9395
  Illegal AgeI site found at 659
  Illegal AgeI site found at 3590
  Illegal AgeI site found at 6298
  Illegal AgeI site found at 8293
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 2938
  Illegal BsaI site found at 3611
  Illegal BsaI site found at 4202
  Illegal BsaI site found at 5368
  Illegal BsaI.rc site found at 2772
  Illegal BsaI.rc site found at 5522
  Illegal BsaI.rc site found at 7807
  Illegal SapI.rc site found at 3302
  Illegal SapI.rc site found at 3848
  Illegal SapI.rc site found at 7699