RNA

Part:BBa_K1723003:Design

Designed by: Emilie Cuillery   Group: iGEM15_EPF_Lausanne   (2015-09-15)
Revision as of 15:11, 15 September 2015 by E.Cuillery (Talk | contribs) (→‎Design Notes)


sgRNA Z4 expressing cassette


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 185
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 185
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 126
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 185
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 185
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part was design on the model for sgRNAs on the paper from Alec AK Nielsen & Christopher A Voigt [3] and the specific sequence for the gRNA Z4 was designed from the sequence of the plasmid pWJ89 D.Bikard [1] sent us.

this part contains a deletion, the 15th base of the terminator is missing. The original sequence is ATTGCAACTTGAAAAAGTGGCACCGAGTCGGTGCTTTTTTT. All of our tests were performed with the deletion and it didn't affect the sgRNA function.

Source

This sequence was fully synthesized.

References