Part:BBa_K1597000
Salt inducible promoter for B. subtilis
Bacillus subtilis is capable of coping with fluctuating salt concentrations. One of the genes involved is proH, this is an 1-pyrroline-5-caboxylate reductase. A study has shown that this gene is under control of the proH-promoter. The proH promoter can be activated by a range of salt concentrations, respectively ranging from 0,1M salt up to 1 M. We have cloned this promoter out of the genome of Bacillus subtilis and combined it with tasA (a gene used in forming the amyloid-like fibers of the biofilm matrix) and GFP. The latter was used to validate the promoter.
We have cloned this promoter out of the genome of Bacillus subtilis 168 and combined it with the tasA gene (produces amyloid-like fibers) from B. subtilis. Both B. subtilis ComI strain with salt promoter and tasA construct (TASA) and B. subtilis ComI (COMI) were grown on Msgg media with and without salt. After 24 hours thioflavin S (which is an amyloid fiber staining) was added to the biofilms. the biofilms were photographed with white light and fluorescence after 15 min incubation.
With the induction of salt, TASA shows more amyloid fibers are present after 24 hours.This is not the case for COMI. This means that the salt promoter is activated with salt and produces extra tasA, resulting in a more intense dye.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 48
- 1000COMPATIBLE WITH RFC[1000]
| None |