Composite

Part:BBa_K1696014

Designed by: Yue Liu   Group: iGEM15_TJU   (2015-09-14)
Revision as of 14:31, 17 September 2015 by Ggjyliuyue (Talk | contribs)

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Mf-ssrA Tag1+linker

We added a short linker after tag1(BBa_K1696013) to increase the original expression level of degraded protein.

Mf-ssrA tag1 is the parental tags of all other variants that are able to be degraded by mf-Lon. In our experiment, we test the proteolysis characteristic of this tag by adding an degraded protein. However, the expression level of the protein is limited due to structural interference between mf-ssrA tag1 and degraded protein. Inspired by that, we further introduce a short linker after tag1, which can disturb the interference and make sure the protein can express in the right way.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
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