Part:BBa_K283047
T7 RNA polymerase
This part codes for the T7 RNA polymerase.This part does not pose any biological threat.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1693
Application
Group: FAFU-CHINA
Author: Ruicheng Dai & Changlong Lu
Summary: The inductive effects of IPTG in different concentration and time lengths
IPTG inductive effect
In our project,we built recombinant plasmids and transformed them into brewer yeast to produce dsRNA. Because of the lack of T7 RNA promoter, we transformed a plasmid which contained T7 RNA polymerase gene. And IPTG could induce the expression of T7 RNA polymerase gene. We tested the IPTG inductive effect in T7 RNA polymerase. In our project,we built the yeast which contained two parts to produce dsRNA. Meanwhile,IPTG could induce the operation of T7 RNA polymerase.Therefore,we could test the concentration of dsRNA to estimate the inductive effect. After the test of IPTG induction,we firmly believed that we could make use of the best IPTG concentration and induction time lengths to get the minimum cost in agriculture.
The inductive effect of different concentration IPTG
We tested dsRNA concentration with the induction of 0.1,0.2,0.3,0.4,0.5,0.6 mmol/L IPTG after 4 hours
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The inductive effect of different time length
We tested dsRNA concentration with the induction time length from 0 to 9 hours. And we tested three group which involved concentration.
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