Coding

Part:BBa_K283047

Designed by: Shi Lei   Group: iGEM09_HKU-HKBU   (2009-10-13)
Revision as of 11:23, 14 September 2015 by Triplet (Talk | contribs)

T7 RNA polymerase

This part codes for the T7 RNA polymerase.This part does not pose any biological threat.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1693


Improvement

Group: FAFU-CHINA

Author: Ruicheng Dai & Changlong Lu

Summary: The inductive effects of IPTG in different concentration and time lengths

IPTG inductive effect

We tested the IPTG inductive effect in T7 RNA polymerase. In our project,we built the yeast which contained two parts to produce dsRNA. Meanwhile,IPTG could induce the operation of T7 RNA polymerase.Therefore,we could test the concentration of dsRNA to estimate the inductive effect.


The inductive effect of different concentration IPTG

We tested dsRNA concentration with the induction of 0.1,0.2,0.3,0.4,0.5,0.6 mmol/L IPTG after 4 hours FAFU-CHINA PART (1).png

The inductive effect of different time length

We tested dsRNA concentration with the induction time length from 0 to 9 hours. And we tested three group which involved concentration. FAFU-CHINA PART (2).png

[edit]
Categories
//cds/enzyme/rnapolymerase
Parameters
None