Coding

Part:BBa_K283047:Experience

Designed by: Shi Lei   Group: iGEM09_HKU-HKBU   (2009-10-13)
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Applications of BBa_K283047

User Reviews

UNIQb5f20e1ccd4e0abe-partinfo-00000000-QINU UNIQb5f20e1ccd4e0abe-partinfo-00000001-QINU

Group: FAFU-CHINA

Author: Ruicheng Dai & Changlong Lu

Summary: The inductive effects of IPTG in different concentration and time lengths

IPTG inductive effect

We tested the IPTG inductive effect in T7 RNA polymerase. In our project,we built the yeast which contained two parts to produce dsRNA. Meanwhile,IPTG could induce the operation of T7 RNA polymerase.Therefore,we could test the concentration of dsRNA to estimate the inductive effect.


The inductive effect of different concentration IPTG

We tested dsRNA concentration with the induction of 0.1,0.2,0.3,0.4,0.5,0.6 mmol/L IPTG after 4 hours FAFU-CHINA PART (1).png

The inductive effect of different time length

We tested dsRNA concentration with the induction time length from 0 to 9 hours. And we tested three group which involved concentration. FAFU-CHINA PART (2).png