Coding
Part:BBa_K1595030
Designed by: Jonlin Chen Group: iGEM15_Cornell (2015-09-13)
EDA linked to cel5a via GSG linker
T7 promoter + Ribosome binding site + EDA + GSG + NdeI + cel5a + BamHI + 6xHist tag + Terminator. Coding device for KDPG aldolase (EDA), a peptide-stabilizing protein, with a Gly-Ser-Gly linker and protein cel5a.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1965
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 641
Illegal NgoMIV site found at 1038
Illegal NgoMIV site found at 1494
Illegal AgeI site found at 1758 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1755
[edit]
Categories
Parameters
None |