Coding

Part:BBa_K1595029:Design

Designed by: Jonlin Chen   Group: iGEM15_Cornell   (2015-09-13)
Revision as of 02:00, 14 September 2015 by J.Chen (Talk | contribs)

EDA and GSG linker


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 729
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 641
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Use in conjunction with strain-expressing T7 polymerase.

Added GSG so EDA could easily be linked to it's associated peptide in future use.

Source

Synthesized by DNA 2.0

References

EDA: NCBI accession number P0A955