RNA

Part:BBa_K1633003:Experience

Designed by: Chen Xi, Zhou Yu, Jiang Waner, Zhang Peng, Tian Chenfei   Group: iGEM15_NJU-China   (2015-09-13)
Revision as of 11:02, 18 September 2015 by ChenXi (Talk | contribs) (→‎Applications of BBa_K1633003)


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Applications of BBa_K1633003

INTRODUCTION

This part is an artificially designed RNA strand. It serves as an element of the Team NJU-CHINA RNAi module. We use it as the siRNA medicine to downregulate the expression of Mu opioid receptor in brain tissue. We designed specific MOR siRNAs based on a free software accessible online. This tool can find the best siRNA sequences on target gene MOR to insure the maximum gene-specificity and silencing efficacy. This tool also designs the pair of oligonucleotides needed to generate short hairpin RNAs (shRNAs) in the plasmid. Then we synthesize the shRNA sequences with the help of a DNA synthesis company. We totally got four such shRNA plasmids.Although MOR siRNA-1 plasmid can efficiently knock down MOR expression, it does not show best interference efficiency and therefore serve as a backup.

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User Reviews

UNIQ99b6f5d51520fc6f-partinfo-00000000-QINU UNIQ99b6f5d51520fc6f-partinfo-00000001-QINU