Coding

Part:BBa_K1602009

Designed by: Leon Kraus, Jonathan Pletzer-Zelgert, Jonas Sindlinger, Carmen Sakas Gandullo, Sebastian Holzner, Elena Tonchevska, Mirjam Haun   Group: iGEM15_TU_Darmstadt   (2015-09-11)
Revision as of 09:26, 14 September 2015 by Henklem (Talk | contribs)

cis-Aconitate decarboxylase - cadA

cadA is a gene, coding for a cis-aconitate decarboxylase in Aspergillus terreus. The dehydrogenase catalyzes the conversion from cis-aconitate to itaconic acid. The reaction takes place in the cytosol of the host.
Figure 1 cis-aconitate decarboxylase (coded by cadA)

Characteristics


Molecular Weight 52754.05
Residues 490
Charge -10.0
Isoelectric Point 5.1050
A280 Molar Extinction Coefficients 59360 (reduced) 60110 (cystine bridges)
[Data taken from PEPSTATS]

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Unknown
  • 12
    INCOMPATIBLE WITH RFC[12]
    Unknown
  • 21
    INCOMPATIBLE WITH RFC[21]
    Unknown
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]
[edit]
Categories
//awards/part_collection/2015
Parameters
None