Coding
ProGlgBRv3

Part:BBa_K1618023

Designed by: Mark Elms Farhan Mitha   Group: iGEM15_NRP-UEA-Norwich   (2015-09-03)
Revision as of 12:10, 21 September 2015 by Nicolapatron (Talk | contribs)

GlgB-Rv3030 with IPTG-inducible promoter

K1618023 contains the coding sequences for GlgB from E. coli and Rv3030 from Mycobacterium tuberculosis (strain ATCC 25618). GlgB cleaves alpha-1,4 linkages in glycogen chains and transfers the cleaved branch onto the main glycogen chain using an alpha-1,6 linkage to make a branch point. Rv3034c is a putative S-adenosylmethionine-dependent methyltransferase. This part will potentially make branched, acetylated glycogen.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 283
    Illegal BamHI site found at 1491
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 917


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Categories
Parameters
None