Coding
GlgB

Part:BBa_K1618000

Designed by: Mark Elms Farhan Mitha   Group: iGEM15_NRP-UEA-Norwich   (2015-08-10)
Revision as of 14:10, 9 September 2015 by Cym13puu (Talk | contribs)

GlgB without inducible promoter

GlgB codes for an enzyme that increases glycogen branching in glycogen. The enzyme cleaves alpha-1,4 linkages in glycogen and transfers the cleaved branch back onto the main glycogen strand using an alpha-1,6 linkage. This creates the branch. The genetic sequence is inserted into the pSB1C3 and therefore displays chloramphenicol resistance.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 57
    Illegal BamHI site found at 1265
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 691


[edit]
Categories
Parameters
None