Composite

Part:BBa_S03642:Design

Designed by: Karmella Haynes   Group: iGEM06_Davidson   (2006-12-14)
Revision as of 03:09, 31 January 2007 by Kahaynes (Talk | contribs) (→‎Source)

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(-2,-1) HixC-TetB-RBSrev-HixC : pBadrev-HixC


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1077
    Illegal NheI site found at 1291
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 931
    Illegal BamHI site found at 1351
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 377
    Illegal NgoMIV site found at 537
    Illegal NgoMIV site found at 905
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

One of the eight permutations of two adjacent hixC-flanked "flippable" parts: pBad and RBS-Tet. This permutation has a reversed RBS-Tet coding region followed by a reversed pBad promoter. This part is maintained in the insulated vector pSB1A7 so that tetracylcine resistance expression is driven solely by the pBad promoter. This part conveys tetracylcine resistance at 50 ug/mL.

Source

BBa_S03641 (HixC-Tetrev-HixC) was cloned into a pSB1A2 vector carrying

References