Composite
Part:BBa_K1660004
Designed by: Dai Yuanyi Group: iGEM15_BNU-CHINA (2015-09-07)
PcyA+Ho1 Synthesis PCB, which can form chromophore with Cph8.
In order to work,Cph8 has to form chromophore with PCB biosynthetic genes, where the formation of PCB requires the gene pcyA and hol to function as accounted below. Hol is a sort of Iron red pigment oxidase which can oxidize the heme group using a ferredoxin cofactor, generating biliverdin IXalpha. And then, PcyA, a kind of ferredoxin oxidase from Synechocystis Sauv, functions to turn biliverdin IXalpha (BV) into PCB. We connected gene pcyA(BBa_I15009)and ho1(BBa_I15008)together along with the constitutive promoter(BBa_J23100) to ensure a continuous work of the photoinduction system in our E.coli.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 816
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 369
- 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
//cds/enzyme
//function/biosynthesis/phycocyanobilin
//function/coliroid
//function/sensor/light
//function/biosynthesis/phycocyanobilin
//function/coliroid
//function/sensor/light
direction | Forward |
tag | None |