Device

Part:BBa_K1679016:Design

Designed by: Cun Wei   Group: iGEM15_OUC-China   (2015-09-07)
Revision as of 20:16, 16 September 2015 by C.Leung (Talk | contribs) (→‎Design Notes)


Promoter+RNA thermometer (ROSE)+mRFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 700
    Illegal AgeI site found at 812
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We use a RNA thermometer to make the device temperature-sensitive. When the temperature is below 42°C,the secondary structure of the RBS after transform would be folded, so that the ribosome can't translated RFP. When the temperature rises above 42°C, the secondary structure of the RBS after transform would be unfolded and produce RFP.

Source

BBa_J23119 BBa_K115001 BBa_E1010

References