Coding

Part:BBa_K1724002

Designed by: Yanrui Ye   Group: iGEM15_SCUT   (2015-09-05)
Revision as of 08:04, 15 September 2015 by Firedfish (Talk | contribs)

MerR

The mercury –sensing regulatory protein, MerR(wild type), which regulates mercury resistance operons in Gram-negative bacteria, is subjected to directed evolution in an effect to generate a MerR mutant that responds to Cadmium ion but not mercury.That is, the MerR mutant is the cadmium-sensing regulatory protein. To get MerR mutant, Oligonucleotide-directed mutagenesis is used to introduce random mutations into the key metal-binding regions of MerR. Finally, Getting the generated Cd-specific MerR mutants appears to be unique.

The quantification data of MerR/CadA operon can be seen in BBa_K1724000.[[1]]

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 258
    Illegal AgeI site found at 360
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None