Coding

Part:BBa_K1699005:Design

Designed by: Emil Ruvinov   Group: iGEM15_BGU_Israel   (2015-08-09)
Revision as of 19:20, 5 September 2015 by Shalevgo (Talk | contribs) (→‎Design Notes)

gRNA for dCas9-VP64 targeting synthetic activation promoter pMLPm under U6 promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 250
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

gRNA in SaCas9 scaffold under U6 promoter. Designed to complement 3 sites on MLP synthetic promoter (9).
Cloned into pSBC13 using EcoRI and PstI restriction sites.
F. tagtcatggcggccgcgtcgacG
R. actgacatgcggccgcttaattaaC

Source

Source

References

9. Tunable and Multifunctional Eukaryotic Transcription Factors Based on CRISPR/Cas http://pubs.acs.org/doi/full/10.1021/sb400081r