Regulatory

Part:BBa_K1720002:Design

Designed by: Yuanbin Cui, Yaran Zhao   Group: iGEM15_SCUT-China   (2015-08-25)
Revision as of 02:33, 18 September 2015 by TommyCui (Talk | contribs) (→‎Design Notes:)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)


Design Notes:

We cloned this basic part into a psb1c3 vector.We designed a hypoxia responsive CMV promotor by inserting a hypoxia responsive element （HRE） to CMV promotor.The hypoxia-responsive element is a minimalcis-regulatory element mediating transactivation by the hypoxia-inducible factor (HIF) in mammalian.So that this part only applies to mammalian organism.

References

1.Rational design of minimal hypoxia-inducible enhancers Stefan Kaluz*, Milota Kaluzová, Eric J. Stanbridge Department of Microbiology and Molecular Genetics, Medical Science I, B210, University of California at Irvine, College of Medicine, CA 92697-4025, USA