Coding
BD-CRY2

Part:BBa_K1592005:Design

Designed by: Shuyan Tang   Group: iGEM15_HUST-China   (2015-09-03)
Revision as of 04:35, 17 September 2015 by WangjieLiu (Talk | contribs)

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GalBD-CRY2 Fusion for Yeast-Two-Hybrid


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 849
    Illegal BglII site found at 1308
    Illegal BamHI site found at 1793
    Illegal XhoI site found at 218
    Illegal XhoI site found at 445
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 733
    Illegal AgeI site found at 1462
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 137
    Illegal BsaI site found at 1085
    Illegal BsaI.rc site found at 494
    Illegal SapI.rc site found at 602


Design Notes

No


Source

Part cloned from pRMH120 of Addgene.

References