DNA
Rluc

Part:BBa_K1722005

Designed by: Kexin Li   Group: iGEM15_SZU_China   (2015-08-29)
Revision as of 05:33, 3 September 2015 by How (Talk | contribs)



Rluc can express Renilla luciferase(Rluc).

Rluc can express Renilla luciferase which has become popular as a reporter enzyme for gene expression assays. Renilla luciferase(RLUC) is a blue-light emitting luciferase of marine anthozoan Renilla reniformis.[1] As a reporter gene, researchers attach it to a regulatory sequence of another gene of interest in bacteria, cell culture, animals or plants. RLUC is chosen as a reporter because the characteristic it confer on organisms expressing it is easily identified and measured. The bioluminescence of the sea pancy, is under the control of a nerve network[2-4] and is stimulated by changes of intracellular Ca2+ concerntration.[5-7] RLUC catalyzes the oxidation of coelenteramide, CO2 and light(480nm),as in the following scheme.

2015 SZU-iGEM construct Rlu with one codon being amber mutated and SV40, the promoter in the same plasmid to introduce Rluc into our system. This plasmid with Rluc, together with two other plasmids, are inserted into the cell. Only when the two promoters in plasmids without Rluc are activated simultanuously to express the downstream DNA sequence can Rluc being translated completely. RLUC that is produced inside the cells catalyzes a reaction with luciferin to produce light. By measuring the light intensity using Luminometer, we can tell the working efficiency of our system under different conditions.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 547


[edit]
Categories
//function/reporter/light
Parameters
None