Signalling

Part:BBa_K1800001:Design

Designed by: Laura Lee Irvin   Group: iGEM15_Georgia_State   (2015-08-28)
Revision as of 21:29, 1 September 2015 by Jwhitley5 (Talk | contribs) (→‎Design Notes)

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Alpha-Factor Secretion Signal


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 244
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part was constructed with the alpha secretion signal coding sequence taken from a pGAPza vector. RFC 25 prefix and suffix were added to enable the creation of fusion proteins that can be secreted outside the cell.

Source

The coding sequence for this part was taken from the pGAPza pichia pastoris expression vector.

References