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Part:BBa_K1621007:Design

Designed by:   Group: iGEM15_Freiburg   (2015-08-29)
Revision as of 13:09, 29 August 2015 by RamonaE (Talk | contribs) (→‎References)


anti-dihydroxyacid dehydratase


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

At position 15 a base change from guanine to thymine was inserted by site-directed mutagenesis to remove the recognition site for PstI without causing a change in the amino acid sequence.


Source

The part's sequence as well as an expression plasmid carrying a His-tagged variant was obtained from the group of Prof. Dr. Hust (TU Braunschweig).

References

Meyer T, Schirrmann T, Frenzel A, Miethe S, Stratmann-Selke J, Gerlach GF, Strutzberg-Minder K, Dübel S, Hust M: Identification of immunogenic proteins and generation of antibodies against Salmonella Typhimurium using phage display (2012). BMC Biotechnology 12:29.