Composite

Part:BBa_K1598002:Design

Designed by: Marta Napiorkowska   Group: iGEM15_UCL   (2015-07-22)
Revision as of 20:04, 27 August 2015 by Martanap (Talk | contribs) (→‎Design Notes)

Lac induced expression cassette + TPH1 (tryptophan hydroxylase 1)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 126
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Source

- Lac induced expression cassette (BBa_K314103) was transformed from 2014 iGEM distribution

- TPH1 human gene was codon optimized for E.coli, synthesized and submitted as BBa_K1598001

- parts were digested with S&P and X&P respectively, run on agarose gel, extracted, purified and ligated together
Source

DNA synthesis

References