Device

Part:BBa_K1720003:Design

Designed by: Ying Guan, Yuanbin Cui   Group: iGEM15_SCUT-China   (2015-08-27)
Revision as of 08:09, 4 September 2015 by TommyCui (Talk | contribs) (Design Notes)


Human phosphodiesterase 5A gene silencing device NO.1


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 273
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 247
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Source

We designed the shRNA according to the homology segment sequence of PDE5A from NCBI

References