Protein_Domain

Part:BBa_K1110003:Design

Designed by: Lydia Studdard   Group: iGEM13_Georgia_State   (2013-09-18)
Revision as of 17:03, 31 August 2015 by Lirvie (Talk | contribs) (→‎References)

Mambalgin-1 cDNA


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

For the design, we used assembly standard 25 and removed a start codon from the sequence. Using the pGAPZα expression system, the GSU team has been working to express the protein in Pichia Pastoris. The pGAPZα vector includes an alpha secretion, MYC epitome, and 6xHis tag region. This sequence is optimized for this expression system.

Source

Dendroaspis polylepis Synthesized by IDT

References

Sequence from Diochot, Sylvie, et al. "Black mamba venom peptides target acid-sensing ion channels to abolish pain." Nature 490.7421 (2012): 552-555. Synthesized by IDT