Coding

Part:BBa_K1607010:Design

Designed by: Jiazheng Xing   Group: iGEM15_Shenzhen_SFLS   (2015-08-23)
Revision as of 05:05, 24 August 2015 by VictorX (Talk | contribs) (→‎Design Notes)


The coding sequence of the SCFV of anti-p185her2/neu antibody chA21


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 370
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


The termination codon: 

   Since it was originally designed for 3'his-tag vectors, this coding sequence does not have a termination codon.

Source

The obtainment of DNA sequence: 

  To get this coding sequence, we found the AA sequence of this SCFV on NCBI first (PDB: 3H3B_B). The AA sequence was then reverse translated into DNA sequence. The DNA sequence was analyzed by gene2oligo and 36 oligos were given as a result. We synthesized the 36 oligos and obtained the 601bp fragment of SCFV coding sequence by LCR assembly using these oligos.


References