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Part:BBa_K1758100:Design

Designed by: Team Bielefeld-CeBiTec 2015   Group: iGEM15_Bielefeld-CeBiTec   (2015-08-21)
Revision as of 00:29, 22 August 2015 by Mrfreeze (Talk | contribs) (→‎Design Notes)


Translation enhancing 5-UTR containing g10-L RBS


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We derived this sequence from the literature, searching for the best suited enhancing sequence for in vitro translation. Our main literature sources were [http://www.ncbi.nlm.nih.gov/pubmed/2676996 Olins et al. 1989], [http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3161345/ Shin and Noireaux 2010], [http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3128707/ Zawada et al. 2011], [http://www.ncbi.nlm.nih.gov/pubmed/23654270 Lentini et al. 2013] and [http://www.ncbi.nlm.nih.gov/pubmed/23927491 Takahashi et al. 2013].

Source

This part was created by applying Gibson primers with suitable overhangson pSB1C3.

References