Composite

Part:BBa_K1604020:Design

Designed by: Elisa Godino   Group: iGEM15_UNITN-Trento   (2015-08-18)
Revision as of 17:54, 18 August 2015 by Eli (Talk | contribs) (→‎Source)

araC-pBAD + beta-carotene


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1144
    Illegal BamHI site found at 3185
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 2721
    Illegal NgoMIV site found at 2851
    Illegal AgeI site found at 979
    Illegal AgeI site found at 1936
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 961


Design Notes

Standard assembly between BBA_K731201 and a PCR amplification of BBa_K274200. BBa_K274200 was extracted by the 2012 distribution kit in pSB1A2 and it was amplified by PCR to restore a missing SpEI site.


Source

Arabinose inducible araC-pBAD promoter come from E. coli genome. Gene coding sequences come from Pantoea ananatis (formerly Erwinia uredovora) genome.

References