Translational_Unit

Part:BBa_K1604030:Design

Designed by: Riccardo Corbellari   Group: iGEM15_UNITN-Trento   (2015-08-18)
Revision as of 17:57, 9 September 2015 by Corbi (Talk | contribs) (→‎References)

pncB

PncB encodes for the enzyme NAPRTase (nicotinic acid phosphorbosyl-transferase). It catalyzes the formation of nicotinate mono-nucleotide, a direct precursor of NAD, from NA.

Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1106
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

pst1 illegal site has been removed from the sequence


Source

Escherichia Coli Bl21 (DE3)

References

  1. <p>Susana J. Berrıos-Rivera, K.-Y. San, and G. N. Bennett. (2002) Metabolic Engineering 4, 238–247</p>
  2. <p>Marcel G. Wubbolts, Peter Terpstra, Jan B. van Beilen, Jaap Kingma, H. A. Rene Meesters, and Bernard Witholt. (1990) JBC, 265 (29), 17665-72</p>
  3. <p>Ka-Yiu San, George N. Bennett, Susana J. Berríos-Rivera, Ravi V. Vadali, Yea-Tyng Yang, Emily Horton, Fred B. Rudolph, Berna Sariyar, and Kimathi Blackwood (2002) Metabolic Engineering, 4, 182–192

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