Regulatory

Part:BBa_K310004:Experience

Designed by: Francis Lee   Group: iGEM10_UIUC-Illinois   (2010-09-15)
Revision as of 17:01, 18 August 2015 by Sarahwideman (Talk | contribs) (→‎Applications of BBa_K310004)

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Applications of BBa_K310004

This part doesn’t work. The part is not the described MicF target, rather it appears to be a Cu-responsive transcriptional regulator from Salmonella enterica.

Restriction analysis with EcoRI and PstI shows an insert that is much larger than the expected 190 bp.

Restriction Analysis

Left: restriction analysis gel of K310004 in pSB1C3. Middle: Simulated gel (SnapGene) with K310004 sequence provided by iGEM Team UIUC Illinois 2010. Right: Simulated gel (SnapGene) with sequence provided by iGEM Team Stockholm 2015

Well 2: Positive control (134 bp) Well 3: K310004 in pSB1C3 digested with EcoRI Well 4: K310004 in pSB1C3 digested with EcoR and PstI Well 5: K310004 in pSB1C3 digested with EcoRI Well 6: K310004 in pSB1C3 digested with EcoR and PstI

We therefore sequenced the part and found that it is a 684 bp BioBrick flanked by the BioBrick prefix and suffix. BLAST alignment of the part showed that it probably is a Cu-responsive transcriptional regulator from Salmonella enterica.

User Reviews

UNIQ1f2b2fc19d3b53ba-partinfo-00000000-QINU UNIQ1f2b2fc19d3b53ba-partinfo-00000001-QINU