Part:BBa_K1699003

gRNA for dCas9-VP64 targeting synthetic activation promoter pMLPm

Ribozyme flanked gRNA compatible for dCas9-VP64. gRNA sequence complements 3 different loci in the synthetic promoter pMLPm, and gRNA dCas9 complex can promote transcription downstream of syntetic promoter (9). It has a hammerhead ribozyme on its 5' and an HDV ribozyme on its 3' end. Upon transcription the ribozymes should cleave the mRNA at specific locations to release the mature gRNA (6).

Usage and Biology

usage and biology

Characterization

characterization

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 144
  Illegal NgoMIV site found at 173
  
• 1000
  COMPATIBLE WITH RFC[1000]

References

6. Self-processing of ribozyme-flanked RNAs into guide RNAs in vitro and in vivo for CRISPR-mediated genome editing http://onlinelibrary.wiley.com/doi/10.1111/jipb.12152/full

9. Tunable and Multifunctional Eukaryotic Transcription Factors Based on CRISPR/Cas http://pubs.acs.org/doi/full/10.1021/sb400081r