Part:BBa_K1638018

hTrx-based scaffold fused to T18 through a flexible linker

This composite part consists of the hTrx-scaffold (used for presenting peptide aptamers) fused to the T18 domain of CyaA (used in the bacterial two-hybrid system). This part is used in bacterial two-hybrid screening for peptide aptamers against specific targets. When a target candidate is fused to the T25 domain of CyaA, and a peptide aptamer binds the target, the T18 and T25 domain is brought into proximity of one another and the formation of cAMP is induced. When combined with a cAMP reporter system, like a system promoting the transcription of RFP, the presence of red-flourescent bacteria indicates an interaction between the peptide aptamer and the target protein.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 624
  Illegal XhoI site found at 753
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 165
  Illegal NgoMIV site found at 575
  Illegal AgeI site found at 381
  
• 1000
  COMPATIBLE WITH RFC[1000]