Measurement
Part:BBa_K1783003
Designed by: Iowis Zhu Group: iGEM15_UMaryland (2015-08-14)
Unstable RFP under Hok/Sok Regulation
Produces unstable RFP under a constitutive promoter. This construct carries the hok/sok toxin/antitoxin (TA) plasmid maintenance system and was used to measure the ability of this alternative plasmid maintenance system to keep plasmids within the cell without the use of antibiotics.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 587
Illegal NheI site found at 610 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 1199
Illegal AgeI site found at 1311 - 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |