Composite

Part:BBa_K1638018:Design

Designed by: Jens Sivkr Pettersen   Group: iGEM15_SDU-Denmark   (2015-08-12)
Revision as of 09:35, 18 September 2015 by Jpettersen (Talk | contribs) (→‎Design Notes)


hTrx-based scaffold fused to T18 through a flexible linker


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 624
    Illegal XhoI site found at 753
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 165
    Illegal NgoMIV site found at 575
    Illegal AgeI site found at 381
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part was synthesized by IDT as a gBlock gene fragment.


Library In oder to use this part for peptide aptamer screening, a random nucleotide library has to be inserted into the scaffold. This library codes for a random peptide library that enables screening of a diverse set of peptide aptamers with different specificity. Typically, the library consist of 20 sets of the codon 'NNK' (where N is A,T,G or C and K is G or C). When designing you library, it should be flanked by two XhoI res. sites. This enables insertion into the xhoI res. site intended for the library insertion.

Important note: This part cannot be used in the standard backbones pSB1C3 or pSB1K3, as they include a xhoI restriction site. Instead we recommend using the pSB1A2 backbone

Source

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References