Composite
Part:BBa_K1582004
Designed by: Chenmao Wang Group: iGEM15_Tianjin (2015-08-06)
Revision as of 09:05, 7 August 2015 by ClarenceWang (Talk | contribs)
GFP+sJanus Fusion Protein
HFBI is a modle of class I of hydrophobins, which are naturally produced by filamentous fungi. Hydrophobins are amphipathic, which means they have the hydrophobic part and hydrophilic part at the same time. HFBI is a kind of hydrophobins and it has magic properties. We combine GFP with HFBI by a linker and make them become a fusion protein. This fusion protein could be used in aqueous two-phase systems to make itself seperated frome mixture. Meanwile, hydrophobin has a property that it can generate a membrane on the surface of solid automatically, which is called self-assembly. We can get a fluorescent protein' membrane by it.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 976
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 644
Illegal SapI site found at 985
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Categories
Parameters
None |