Composite

Part:BBa_K1638009

Designed by: Jens Sivkr Pettersen   Group: iGEM15_SDU-Denmark   (2015-05-19)
Revision as of 12:30, 23 July 2015 by Jpettersen (Talk | contribs)

T18 domain of cyaA from Bordetella pertussis with GFP reporter

T18 domain of the catalytic active domain of CyaA from Bordetella pertussis linked together with GFP through a flexible 10 aa linker. This indirectly allows detection of correctly transcription, translation and folding of both T18 domain and GFP (by the presence of green fluorescence).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 711
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 252
    Illegal NgoMIV site found at 662
    Illegal AgeI site found at 468
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1384


[edit]
Categories
Parameters
None