Translational_Unit
Part:BBa_K1033120
Designed by: Magnus Bergman, Pontus Danielsson, Alexander Wirtanen, Nils Anlind, Christoffer Frisk, Thorsteinn Olaufsson, Malin Borg, Niclas Sandberg Group: iGEM13_Uppsala (2013-09-02)
B0034-ExpTag-Linker-Miraculin-Linker-6xHis
Construct needed to produce the protein miraculin, responsible for the taste-altering effects of the berries from the plant "Synsepalum Dulcificum".
This part is a synthetic construct, fitted with an export tag for transportation out of the cell, and a histidine tag (6x His) for purification purposes.
Applications
Miraculin can be used as a sweetener.
Characterization data from Uppsala iGEM 2013
We were not sucessful in either assembling or mutagenizing a promoter to the construct, and as such could not characterize it further.
Registry Curation
Original sequence changed by iGEM HQ
- Change - "TAT" added to start of part sequence, notified by Team [http://2015.igem.org/Team:UMaryland UMaryland 2015 Team]
- Reason - To reflect sample sequence
- Change - Part type from "Coding" to "Translational Unit"
- Reason - RBS in front of coding Region
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
None |