Protein_Domain

Part:BBa_K1638006

Designed by: Jens Sivkr Pettersen   Group: iGEM15_SDU-Denmark   (2015-05-10)
Revision as of 10:14, 9 July 2015 by Jpettersen (Talk | contribs)

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10 aa linker with BamHI restriction site and TEV recognition site

Flexible 10 amino acids linker with the sequence GSENLYFQSG. The linker enables fusion between two proteins. The linker also includes the Tobacco Etch Virus (TEV) protease recognition site, ENLYFQS. This enables separation of the two proteins linked together. In order to prevent scarsites that encodes stop-codons(from the standard assembly RFC[10]), this part also includes a BamHI site (see design page for further description).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None