Regulatory
pLac

Part:BBa_K1638000

Designed by: Jens Sivkr Pettersen   Group: iGEM15_SDU-Denmark   (2015-05-07)
Revision as of 12:59, 8 July 2015 by Jpettersen (Talk | contribs)

Lac operator

A regulator sensitive to the Lac Repressor (encoded by the regulator gene LacI) and the catabolite activator protein (CAP).

The regulator contains two binding sites. The first binds the CAP protein, which is generally present in E. coli and is asocciated with cell Health and availbaility of glucose. The second binds Lacl protein.

In the absence of Lacl protein and CAP protein, this part promotes transcription. In the presence of LacI protein and CAP protein, this part inhibits transcription. LacI can be inhibited by IPTG. LacI is coded by BBa_C0010.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None