Regulatory

Part:BBa_J119376:Design

Designed by: Anthony Eckdahl   Group: Eckdahl Lab   (2015-03-21)
Revision as of 13:13, 27 March 2015 by Eckdahl (Talk | contribs) (→‎Design Notes)


Psimp1 Promoter with Four Consensus Variants


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The consensus sequence from De Mey et al. shown below was used to generate a promoter that has a simple sequence by using W=A, N=G, R=G, and D=G, but avoiding runs of 4 or more Gs or Cs. The resulting promoter was named Psimp1.

De Mey 2007 Figure 1.jpg

Source

Synthetic oligonucleotides.

References

De Mey M, Maertens J, Lequeux GJ, Soetaiert WK, Vandamme EJ (2007) Construction and model-based analysis of a promoter library for E. coli: an indispensable tool for metabolic engineering. MC Biotechnol 7:34.