Reporter

Part:BBa_J100198

Designed by: Telavive Taye   Group: Campbell M Lab   (2014-12-01)
Revision as of 15:02, 20 December 2014 by Macampbell (Talk | contribs)

J100198 contains the fitness gene tester construct (J100199) with thymidylate synthase as the fitnes

Cloned in the fitness gene tester construct (Part:BBa_J100199), part J100198 contains the fitness gene thymidylate synthase Part:BBa_J100132. J100198 contains a theophylline riboswitch downstream of T5, a constitutive promoter Part:BBa_J100132. The construct is built to be used in thymine auxotrophs that necessitate added thymine in the growth media for cell division. Upon successful binding of theophylline on the riboswitch (we used 0.3mM), thymidylate synthase is expressed for prototophic growth and cellular division. J100198 was cloned into pSB1A8 Part:BBa_J119043.

See Experience page for details about how well this device works.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1
    Illegal PstI site found at 995
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1
    Illegal PstI site found at 995
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1
    Illegal PstI site found at 995
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1
    Illegal PstI site found at 995
    Illegal AgeI site found at 107
    Illegal AgeI site found at 770
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None