Composite

Part:BBa_K1471008:Design

Designed by: Juan Noe Hernandez Salazar   Group: iGEM14_BIOSINT_Mexico   (2014-10-08)
Revision as of 21:39, 2 November 2014 by Jaxys (Talk | contribs) (→‎Design Notes)

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RBS - PhyB - AGS - VP16.


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part includes the genes for PhyB protein domain, an activation domain and AGS linker which helps in the protein complex formation with PhyB.

Source

Arabidopsis thaliana genome

References

Taiz,L. and E.Zeiger.(2006) Fisiologia Vegetal.Universitat Jaume. 3rd Edition.Pages 7-27 Rangan, et al. (2008). Analysis of Context Sequence Surrounding Translation Initiation Site from Complete Genome of Model Plants. New York University. [Online] Retrieved october 14th 2014 from: http://www.nyu.edu/projects/vogel/Reprints/

Rangan_MolBt08.pdf Nakagawa, et al. (2007). Diversity of preferred nucleotide sequences around the translation initiation codon in eukaryote genomes. Oxford University Press. [Online] Retrieved october 14th 2014 from: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2241899/ 

Liu Q, Xue Q. (2005). Comparative studies on sequence characteristics around translation initiation codon in four eukaryotes. Zhejiang University. [Online] Retrieved october 14th 2014 from: http://www.ias.ac.in/jgenet/Vol84No3/317.pdf

Kozak, M. (1989). Circumstances and mechanisms of inhibition of translation by secondary structure in eucaryotic mRNAs. American Society for Microbiology (ASM). [Online] retrieved october 14th 2014 from: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC363665/

Tiley LS1, Madore SJ, Malim MH, Cullen BR.(1992).The VP16 Transcription Activation Domain Is Functional When Targeted to a Promoter-proximal RNA Sequence. Genes Development. Volume 6; Issue 11; Pages: 2077-2087.

Carbone, A-L, M. Moroni, P-J Groot-Kormelink, and I. Bermudez(2009).Pentameric concatenated (α4)2(β2)3 and (α4)3(β2)2 nicotinic acetylcholine receptors: subunit arrangement determines functional expression. Britain Journal of Pharmacology.Volue 156; Issue 6; Pages 970–981.