Coding

Part:BBa_K1413021:Design

Designed by: Laura MATABISHI, Julie Zaworski   Group: iGEM14_Evry   (2014-10-07)
Revision as of 21:05, 1 November 2014 by Laurabibi (Talk | contribs) (→‎Source)


bphR2 mutated


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 475
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 259


Design Notes

Moreover, the sequence contained a pstI site from 142 to 148bp after ATG site so we have synthetized it by mutating the site, keeping the same amino acid.


Source

The bphr2 gene was synthetized from the sequence Pseudomonas pseudoalcaligenes KF707 DNA genomic without its PstI site. The part bphr2 of 2013 Paris Saclay's team is not available in the registry and in the team.

References

Kensuke Furukawa and Hidehiko Fujihara, Microbial Degradation of Polychlorinated Biphenyls : Biochemical and Molecular Features, 2008. Journal of Bioscience and Bioengineering, Vol. 105, No. 5, 433–449.