Coding

Part:BBa_K1539001:Experience

Designed by: Coleen Tran   Group: iGEM14_GeorgiaTech   (2014-10-09)
Revision as of 20:30, 1 November 2014 by Stassoulas3 (Talk | contribs) (→‎Applications of BBa_K1539001)

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Applications of BBa_K1539001

BBa_K1539001 was created using devices XXX and YYY to insert relatively high expressing promoters and high efficiency RBS sites in front of mCherry from BBa_J0650. Expression of mCherry was quantified by flow cytometry using a FACS AriaIII. Relative to cells transfected with an mCherry plasmid without a promoter or RBS site, 91.6% of our cells expressed mCherry and the median intensity was 4X brighter than a low promoter/weak RBS combination (see part [1]). Our raw data is summarized below:

"High Expression Combination" - Primers PH1/RH1: File:File:///C:/Users/yannis/Downloads/140821 DMC mCherry PH1-RH1.pdf Negative Control:

User Reviews

UNIQ78c0d8fc3a65d500-partinfo-00000000-QINU UNIQ78c0d8fc3a65d500-partinfo-00000001-QINU