Generator
3-MBG

Part:BBa_J45400

Designed by: MIT IGEM 2006   Group: iGEM06_MIT   (2006-08-21)
Revision as of 05:27, 31 October 2006 by Stephen (Talk | contribs)

3-methylbutanal generator

This Isoamyl Alcohol Generating Device converts leucine into 3-methylbutanal (which is then reduced to isoamyl alcohol by NADH). The two coding regions of BAT2 and THI3 are derived from the S. cervisiae leucine degradation pathway.

IAGDactual.jpg


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1250
    Illegal EcoRI site found at 2151
    Illegal EcoRI site found at 2850
    Illegal EcoRI site found at 3036
    Illegal SpeI site found at 1944
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1250
    Illegal EcoRI site found at 2151
    Illegal EcoRI site found at 2850
    Illegal EcoRI site found at 3036
    Illegal SpeI site found at 1944
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1250
    Illegal EcoRI site found at 2151
    Illegal EcoRI site found at 2850
    Illegal EcoRI site found at 3036
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1250
    Illegal EcoRI site found at 2151
    Illegal EcoRI site found at 2850
    Illegal EcoRI site found at 3036
    Illegal SpeI site found at 1944
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1250
    Illegal EcoRI site found at 2151
    Illegal EcoRI site found at 2850
    Illegal EcoRI site found at 3036
    Illegal SpeI site found at 1944
    Illegal AgeI site found at 1114
  • 1000
    COMPATIBLE WITH RFC[1000]


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